Order NO. C610303
重組PreScission酶
| 英文名 | Recombinant PreScission Protease | ||
| 別名 | PPase, Recombinant | ||
| 相關類別 | 酶 | 儲存 | 冷凍（-20℃） |
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| 編 號 | 包裝 | 庫存 | 目錄價（￥） | 您的價格（￥） | 數 量 |
| C610303-0100 | 100 IU | 現貨 |
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產品描述
概述
PreScission protease is a cysteine protease derived from human rhinovirus – HRV3C Protease. rPP is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. It specifically recognizes the amino acid sequences which include the core site of Leu-Phe-Gln-Gly-Pro and cleaves between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the super structures of the fusion protein. rPP works most effective at 4℃ and can digest substrates at room temperature as well.
使用說明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE
屬性
| Physical Appearance | Sterile colorless liquid. |
| Source | Escherichia coli. |
| Recommended Conditions for Cleavage of a Fusion Protein | During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required |
| Cleavage Buffer | 50 mM Tris-HCl, pH 7.0 (at 25°C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5 °C prior to use. |
| Unit Definition | One unit is defined as the amount of enzyme needed to cleave 100 μg of fusion protein in 16 hours to 90 % completion at 5 °C in a buffer containing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT. |
相關文檔
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質量控製相關文件
質量報告 (COA)